Human SCF, premium grade130-096-695
产品说明
Background information
SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule.
Biological activity
Proliferation of TF-1 cells (NIBSC 91/682)
research grade: ≥ 2×105 U/mg
premium grade: ≥ 4×105 U/mg
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
相关图片
Human SCF activity assay. The biological activity of Human SCF is determined by proliferation assay using TF-1 cells.
SDS-PAGE of Human SCF, premium grade under reduced (R) and non-reduced (NR) conditions.
Human SCF biological activity. Activity of Human SCF, premium grade (red bar) was compared to another commercially available product (black bar).
Mass spectrometry analysis (ESI-MS) of Human SCF, premium grade. The peak corresponds to the calculated molecular mass of 18585 Da.