CD1c (BDCA-1)-PE, human (clone: AD5-8E7)130-098-007
产品说明
Blood CD1c (BDCA-1)+ myeloid dendritic cells are CD11chigh, CD123low, CD4+, Lin–, CD45RO+, CD2+, CD16–, CD141 (BDCA-3)low, CD303 (BDCA-2)–, and CD304 (BDCA-4/Neuropilin-1)–. They express myeloid markers (CD13, CD33) as well as Fc receptors (CD32, CD64, FcεRI) and are of monocytoid appearance. A minor proportion of CD1c (BDCA-1)+ myeloid dendritic cells expresses CD14 and CD11b. In blood, CD1c (BDCA-1) is also expressed on a subpopulation of CD19+ small resting B lymphocytes. CD1c (BDCA-1) expression has also been detected on cortical thymocytes, on Langerhans cells, and on CD1a+ dendritic cells generatedex vivo from monocytes or hematopoietic precursor cells. CD1c (BDCA-1)+ myeloid dendritic cells have been designated as type-1 myeloid dendritic cells (MDC1s).
The CD1c antigen is a member of the CD1 family of proteins that are structurally related to MHC class I proteins and mediate the presentation of non-peptide antigens to T cells.
Alternative names
BDCA-1, CD1, R7, R7, M241, T6, CD1a
Clone and isotype
| Clone | Isotype |
|---|
| AD5-8E7 | mouse IgG2a |
Cross reactivity
This antibody is tested to cross-react with
Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)
In blood, CD1c is expressed on rhesus and cynomolgus monkey myeloid dendritic cells and on a subset of CD20+ B cells. The antibody has also been reported to cross-react with CD1c on pigtail monkey (Macaca nemestrina) cells.
相关图片
Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies or with the corresponding isotype control antibodies (left image), as well as with CD19 and CD11c antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Apart from type-1 myeloid dendritic cells (MDC1s), CD1c (BDCA-1) is also expressed on a subset of B cells. For this reason, B cells are excluded from the analysis according to expression of CD19 and further analysis is restricted to CD19– cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
PBMCs from rhesus monkey were stained with CD1c (BDCA-1)-PE and CD20-FITC and analyzed by flow cytometry.
Cynomolgus monkey PBMCs were stained with CD1c (BDCA-1)-PE and CD20-FITC and analyzed by flow cytometry.
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CD1c (BDCA-1)-FITC, human (clone: AD5-8E7) Regulatory status: For research use only Capacity: for 30 tests 130-098-009