GoScript™ 逆转录酶适用于 qPCR，包括与 GoTaq® qPCR 及 Plexor® qPCR 系统联合用于 RT-qPCR。
特点 - 优点
• 超强活性：节约每个反应的成本。
• 灵敏：可检测低丰度转录本。
• 持续性好：能转录长 mRNA。
• 适应性强：强抑制剂存在时也能合成 cDNA。
应用
• RT-qPCR
• RT-PCR
注意事项
GoScript™ Reverse Transcription System includes: GoScript™ Reverse Transcriptase, GoScript™ 5X Reaction Buffer, MgCl2, PCR Nucleotide Mix, Oligo(dT)15Primer, Random Primers, Nuclease-Free Water and Recombinant RNasin® Ribonuclease Inhibitor.
GoScript™ Reverse Transcriptase includes GoScript™ Reverse Transcriptase, GoScript™ 5X Reaction Buffer and MgCl2.
GoScript™ Reverse Transcription Mix, Oligo(dT), includes: GoScript™ Enzyme Mix (GoScript™ Reverse Transcriptase and RNasin® Plus RNase Inhibitor), GoScript™ Reaction Buffer, Oligo(dT) (dNTPs, MgCl2 and Oligo (dT)15 Primer) and Nuclease-Free Water.
GoScript™ Reverse Transcription Mix, Random Primers, includes GoScript™ Enzyme Mix (GoScript™ Reverse Transcriptase and RNasin® Plus RNase Inhibitor) and GoScript™ Reaction Buffer, Random Primers (dNTPs, MgCl2 and Random Primer) and Nuclease-Free Water.
Figure 1. Reverse transcription of an 8.9kb APC transcript.
Human colon total RNA (Clontech Laboratories, Inc.) was reverse transcribed using oligo(dT) (O) or random primers (R) according to the manufacturer’s instructions for each reverse transcriptase. cDNA then was analyzed by endpoint PCR using GoTaq® Green Master Mix (Cat.# M7122) with primers for a 940bp amplicon at the 5´ end of the transcript. Each reaction (5µl) was analyzed by agarose gel electrophoresis and ethidium bromide staining. All samples shown were analyzed in the same data set and gel with the same scan settings. Gel images are representative of results from three separate experiments.