服务热线: 010-82349466
产品中心 Products
联系我们
  • 传真:010-82349418
  • 电话:010-82349466/9931/9293
  • 服务邮箱:order@ulab360.net



GoTaq® Real-Time qPCR and RT-qPCR Systems for Dye-Based Detection
产品名称:

GoTaq® Real-Time qPCR and RT-qPCR Systems for Dye-Based Detection

上市日期: 2017-01-19

The GoTaq® qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.

产品概述

GoTaq® qPCR Master Mix 5ml A6001

GoTaq® qPCR Master Mix 25ml A6002

GoTaq® 2-Step RT-qPCR System 5ml A6010

GoTaq® 1-Step RT-qPCR System 5ml A6020


The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

特点 - 优点

  • Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.

  • Rely on a Robust System: Resistant to a wide range of PCR inhibitors.

  • Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments.

  • Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.

应用

  • Gene expression analysis.

  • Genotyping.

  • Detection of sequence variants.

  • Sensitive sequence detection and quantification.

注意事项



Number of Reactions
Product Cat.#Size50µl reactions20µl reactions10µl reactions
A60015ml200 reactions500 reactions1,000 reactions
A600225ml1,000 reactions2,500 reactions5,000 reactions
A60105ml50 × 20µl RT reactions and
200 × 50µl qPCR reactions
50 × 20µl RT reactions and
500 × 20µl qPCR reactions
50 × 20µl RT reactions and
1,000 × 10µl qPCR reactions
A60205ml200 reactions500 reactions1,000 reactions


推荐产品 / Products
  • 发布时间: 2017 - 03 - 08
    保护工作人员及病人,免受污秽。一次性使用,全身设计,有手袖,干净卫生。轻盈PE防水物料,加长白色束手袖设计,加强保护性,颈部魔术贴设计,方便穿着。
    8033隔离服,蓝色,复合塑料膜(防水),5袋/箱,10件/袋一次性使用,方便可靠 附头帽设计,覆盖范围大,由头到脚均得到周全的保护由40g聚丙烯 (PP) 无纺布制成,具抗水、抗静电及防尘功能质料坚韧、轻盈及透气胸前附有长拉链,可迅速地穿着或更换手袖、裤脚、腰间及头帽均采用弹性边缘设计,贴身舒适,更有三款不同尺码,适合任何人士穿着
  • 发布时间: 2017 - 03 - 08
    用于: Industrial, Health and beauty, Retail提供: 中国
    8000-B条帽,17cm,蓝,10袋/箱,100只/袋8000-W条帽,17cm,白,10袋/箱,100只/袋弹性围边,可稳固抓紧头发,轻盈无纺布材料制成,透气度高,柔软舒适不含乳胶的弹性头带绳,减少过敏反应8045B条帽在传统条帽的基础上,使用SMS材质,材质更厚、柔软,有一定的阻菌性。
  • 发布时间: 2017 - 03 - 03
    意大利制造,任意弯曲不造成水阻塞700i   蓝色嘴头• 105支/袋 • 10袋/箱
    意大利原装进口品质保证,任意弯曲无死角,定性准确不反弹由防锈合金和优质塑胶材料制成,能够弯成各种需要的造型平滑牢固的尖端管口设计,不会脱落并能防止吸住口腔内的组织,安全舒适。每包105根,多送5根平口尖端设计,不吸肉,牢固管口设计不脱落,安全舒适 防锈合金和优良胶材制成,能夠弯曲所需角度,不反弹
Copyright © 2005 - 2013 北京照生莱博商贸有限公司
犀牛云提供企业云服务