CD4+ Effector Memory T Cell Isolation Kit, human130-094-125
产品说明
Detailed separation procedure
For magnetic depletion of non-target cells, PBMCs are incubated with a cocktail of biotinylated antibodies against CD45RA, CD8, CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, Anti-TCRγ/δ, and CD235a (glycophorin A) as well as APC-conjugated CD197 (CCR7) antibodies. In a second step, all antibody-labeled cells are labeled with MicroBead Cocktail for depletion. The CD4+Effector Memory T Cell Isolation Kit provides a fast and efficient way to isolate untouched CD4+effector memory T cells.
Downstream applications
CD4+ effector memory T cells isolated with the CD4+ Effector Memory T Cell Isolation Kit can be used for functional studies, for studies on signal transduction, or for analysis of cytokine expression.
Columns
LD or autoMACS® Columns.
相关图片
Untouched CD4+ effector memory T cells were isolated from human PBMCs using the CD4+Effector Memory T Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD4-FITC and CD45RO-PE. Cells were labeled with CD197 (CCR7)-APC during the separation procedure.
PBMCs before separation
Gated on CD4+ cells.
Isolated CD4+ effector memory T cells
Gated on CD4+ cells.