Kinase-Glo® Luminescent Kinase Assay 10ml V6711
Kinase-Glo® Luminescent Kinase Assay 10 × 10ml V6712
Kinase-Glo® Luminescent Kinase Assay 100ml V6713
Kinase-Glo® Luminescent Kinase Assay 10 × 100ml V6714
Kinase-Glo® Max Luminescent Kinase Assay 10ml V6071
Kinase-Glo® Max Luminescent Kinase Assay 10 × 10ml V6072
Kinase-Glo® Max Luminescent Kinase Assay 100ml V6073
Kinase-Glo® Max Luminescent Kinase Assay 10 × 100ml V6074
Kinase-Glo® Plus Luminescent Kinase Assay 10ml V3771
Kinase-Glo® Plus Luminescent Kinase Assay 10 × 10ml V3772
Kinase-Glo® Plus Luminescent Kinase Assay 100ml V3773
Kinase-Glo® Plus Luminescent Kinase Assay 10 × 100ml V3774
The assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity. The Kinase-Glo® Assays generate a "glow-type" luminescent signal produced using a patented stabilized luciferase (Ultra-Glo™ Luciferase) coupled with a proprietary buffer system. When assayed in the presence of kinase reaction buffers, such as the reaction buffer for PKA, the half-life of the luminescent output is greater than five hours, eliminating the need for luminometers with injectors and allowing for batch plate processing. The assay produces excellent Z´-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC50 values comparable to those reported in the literature.
The Kinase-Glo® Assay systems are differentiated by their linear response to ATP (see figure below). The original Kinase-Glo® Assay is linear to 10μM ATP, while Kinase-Glo® Plus Assay is linear to 100μM ATP. The newest assay, Kinase-Glo® Max, is linear to 500μM ATP, making it well suited for use with kinases with high Kmfor ATP as well as for screening for kinase inhibitors that do not compete at the ATP binding site.
特点 - 优点
Assay a Variety of Kinases: Can be used for a wide range of kinases (including lipid, sugar and alcohol kinases) and substrates (peptides, proteins, lipids, sugars and alcohols).
Obtain Reliable Results: Luminescence is much less susceptible to interference from library compounds than other luciferase-based ATP detection reagents. Z´-factor values greater than 0.7 in either 96- or 384-well plate formats.
Simplify Your Assay: Homogeneous—everything is performed in a single well.
Non-Radioactive: No radioactive waste disposal and safety issues.
Automate This Assay: Validated automated methods available at: www.promega.com/automethods/
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Screen for Non-ATP Binding Site Inhibitors: Use ATP concentrations as high as 500μM (Kinase-Glo® Max Assay).
储存条件
Store at –20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
Figure 1. Luminescent output correlates with amount of ATP.
A direct relationship exists between the luminescence measured with the Kinase-Glo® Assays and the amount of ATP in the reaction. Panel A. Kinase-Glo® Plus Assay. Panel B. Kinase-Glo® Max Assay. The Kinase-Glo® Assay is linear to 10µM (data not shown).