时间:
2017
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01
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19
Wizard® Plus Maxipreps DNA Purification System 10 preps A7270可单独购买 Cell Resuspension Solution (CRA) 150ml A7112Cell Lysis Solution (CLA) 150ml A7122Neutralization Solution (NSA) 150ml A7131Column Wash Solution (CWB) 125ml A8102Wizard® Maxipreps DNA Purification Resin 500ml A7401Wizard® Maxi/Megapreps Filtering System 50 each A7421该系统通常在不到3个小时内,从100-500ml的培养物中得300μg–1mg的高拷贝质粒DNA(200–20,000 bp)。纯化的质粒可直接用于自动荧光DNA测序及限制酶酶切消化,无需进一步操作,也可用于体外转录反应,此时反应体系中需含有核糖核酸酶抑制剂(如重组RNasin® Ribonuc...