如果你跟全球大药厂的科研人员聊天说到CellTiter-Glo®细胞活力检测系统，他们总会亲切地称他为CTG。这个已经进入大药厂药物研发SOP多年的经典系统，一经推出，便用其超凡的性能征服了用户，并为药物研发的加速做出了不可忽视的贡献。CellTiter-Glo®细胞活力检测系统的魅力到底在哪里，今天就让小麦带你近距离接触一下这个传奇的检测系统。
CellTiter-Glo® Luminescent Cell Viability Assay
简称CTG, 是通过对 ATP 进行定量来测定培养物中活细胞数目的一种快速均质检测方法。ATP 是活细胞新陈代谢的关键指标。与传统的MTT比色法不同，这种基于高灵敏度生物发光方法的检测技术，不受化合物自发荧光的影响，创造了操作时间和检测灵敏度的新高峰。
这是目前任何比色法都无法企及的指标。基于上述特点，这个技术可完美地与高通量操作兼容，也为高通量药筛带来了福音。
综上所述，曾被MTT法虐了多年的小麦不知道为什么有种被亮瞎眼,相!见!恨!晚!的感觉。
下面献上部分CellTiter-Glo®华丽丽的高影响因子文献列表
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NRF2 Promotes Tumor Maintenance by Modulating mRNA Translation in Pancreatic Cancer. Chio, II et al. Cell166, 963-76.
TRIF licenses caspase-11-dependent NLRP3 inflammasome activation by gram-negative bacteria. Rathinam, V.A. et al. Cell150, 606-19.
Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Sun, L. et al. Cell 148, 213-27.
P7C3 neuroprotective chemicals function by activating the rate-limiting enzyme in NAD salvage. Wang, G. et al. Cell 158, 1324-34.
A high-throughput drug screen for Entamoeba histolytica identifies a new lead and target. Debnath, A. et al. Nat Med 18, 956-60.
Proteome-wide covalent ligand discovery in native biological systems. Backus, K.M. et al. Nature 534, 570-4.
Allosteric inhibition of SHP2 phosphatase inhibits cancers driven by receptor tyrosine kinases. Chen, Y.N. et al.Nature 535, 148-52.
Class IIa HDAC inhibition reduces breast tumours and metastases through anti-tumour macrophages. Guerriero, J.L. et al. Nature 543, 428-432.
Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores. Liu, X. et al. Nature 535, 153-8.
Selective transcriptional regulation by Myc in cellular growth control and lymphomagenesis. Sabo, A. et al. Nature 511, 488-92.
YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14. Huang, J.M. et al.Oncogene 32, 2220-9.
Wetterskog, D. et al. Identification of novel determinants of resistance to lapatinib in ERBB2-amplified cancers. Oncogene 33, 966-76.
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Kobayashi, E. et al. MicroRNA expression and functional profiles of osteosarcoma.Oncology 86, 94-103.
Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Lin, H., Lee, E., Hestir, K., Leo, C., Huang, M., Bosch, E., Halenbeck, R., Wu, G., Zhou, A., Behrens, D., Hollenboguh, D., Linnemann, T.,Qin, M., Wong, J., Chu, K., Doberstein, S.K. and Williams, L.T.，Science 320, 807-11.2008
Genome-wide RNAi analysis of growth and viability inDrosophila_cells. Boutros, M., Kiger, A., Armknecht, S., Kerr, K., Hild, M., Koch, B., Haas, S., Heidelberg Fly Array Consortium, Paro, R., and Perrimon, N.Science 303, 832-835.2004
The threshold pattern of calcineurin-dependent gene expression is altered by loss of the endogenous inhibitor calcipressin. Ryeom, S., Greenwald, R.J., Sharpe, A.H. and McKeon, F. Nature.Immunol. 4(9), 874-881.2003
现在为了让大家更好的了解CTG， Promega推出了CTG小包装4.3折的促销活动，让您能轻松与CTG亲密接触，体验它带给你的VIP级实验体验。下面让我们来认识一下这个金牌家族：
这只是个开始哦, Promega近期将推出一系列的关于CTG的活动，请大家拭目以待，不要换台哦！